Yeast Vectors

Yeast, Saaccharomyces cerevisiae, is an eukaryotic with 34 chromosomes. it reproduces sexyally as well as asexually be budding. In suspension cultures it grows as single cells with cell doubling time of 1.5 to 2.5 hrs but on agar plates cells produce colonies. Its haploid DNA content is only times that E. coli and its genetics hasbeen extensively studied. Yeast viruses are not known only a single yeast plasmid has been discovered which has been used to contrast some useful vectors. The tough polysaccharide wall of yeast is an effective barrier to DNA molecules. Therefore, yeast cell wall is enzymatically digested to produced spheroplasts which can take up DNA following treatment with cacl2 walls regenerate the specific media.