The recombinant DNA molecule is produced by joinign together two or more DNA sesgments usually originating from different organisms. More specifically a rcombinant DNA molecule is a vector into which the desired DNA fragment has been inserted to enable its cloning in appropriate host. Thisis achieved by using specific enzymes for cutting the DNA (restriction enzyme) into suitable fragments and then for jointing together the appropriate fragments (ligation) In this manner, a recombinant DNA molecule may be produced which contains a gene from one organism joined to regulatory sequences from another organism, such a gene is called chimaeric gene. CLearly, the capability to produced novel gene combination to suit specific needs. Recombinant DNA molecule produced one of three ways (1) to obtain a large number of copies of specific DNA fragments (2) to recover large quantities of the protein produced by the concerned gene (3)
to integrate the gene in question into the chromosome of a target organism where it express itself.
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