Steps in gene cloning

The entire procedure of cloning or recombinant DNA technology may be classified must the following five steps for the convenience in description and on the basis of the chief activity preformed.
1. Identification and isolation of the desired gene or DNA fragment to be cloned.
2. Insertion of the isolated gen in a suitable vector.
3. Introduction of this vector into a suitable organism or cell called host (transformation).
4. Selection of the transformed host cell, and
5. Multiplication /expression/ integration followed by expression of the introduced gene in the host.
A brief description of these steps is given in the following sections.