Pappilomavirus belong to papova virus class and causes wanrts. They have ciruclar 8 kb genome organised in nucleosomes. Th ebovine papillomavirus replicate as a stable plasmid in rodent and many bovine cells and the cells are killed. The viral genome transforms cells which behave like tumor cells and from piled up colonies of cells instead of the typical monolayer. THe transformed state is due to the genes present in the transforming region (about 5500 bp of the virus genome. The virus genome is generally used to produce shuttle vectors by using the transforming region of viral genome.
Eukaryotic DNA segments are first cloned in E. coli to select recombinant vectors. Then the E. coli plasmid say pBR 322 is deleted from the vectors, and the linear recombinant vector is introduced into animal cells the vector becomes circular and replicates as plasmid. The E.coli neo gene may be included within the vector this allows easy selection of transfected cells by culturing them on medium containing the aminoglycoside G-418.
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